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The use of recombinant DNA and related technologies has revealed the extraordinary degree to which cell cycle control mechanisms have been conserved through eukaryotic evolution. The availability of genes and gene products derived from different organisms has greatly extended what is experimentally possible. As a result, many researchers are now faced with the need to handle a far wider range of organisms and must therefore be acquainted with the ever-increasing arsenal of experimental techniques. This book is a compilation of key techniques currently used in the most popular experimental systems. The book describes how specific organisms or cell types are handled and focuses on particular techniques that are applicable to any cell type. A wide range of biochemical, genetic, and cellular manipulations are described. Students and researchers in cell and molecular biology will welcome this accessible, useful new guide.
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Reversible phosphorylation is one of the major mechanisms by which protein activity is controlled in all eukaryotic cells. Networks of protein kinases and phosphatases, which catalyze phosphorylation and dephosphorylation respectively, are responsible for switching between different pathways of cell division, development, or differentiation in response to hormones or growth factors. This book provides a comprehensive description of current methods for studying protein phosphorylation and the protein kinases and phosphatases which catalyze it. It includes detailed protocols for studying phosphorylation in intact cells, approaches using enzyme inhibitors, purification of serine/threonine-specific protein kinases/phosphatases, analysis of kinase/phosphatase specificity, and cloning relevant cDNAs. Protein Phosphorylation: A Practical Approach will be of great value to all researchers in the fields of intracellular and intercellular signalling, including biochemists, molecular biologists and pharmacologists.
Enzymology --- Phosphoproteins --- Phosphorylation --- Protein kinases --- Phosphoprotein phosphatases --- Metabolism --- Research --- Methodology --- Phosphoprotéines --- Phosophorylation --- Phosphoprotéine phosphatase --- Protéines kinases --- Phosphorylation. --- Protein Kinases. --- Phosphoprotéines --- Phosphoprotéine phosphatase --- Protéines kinases --- Methodology. --- Métabolisme --- Recherche --- Méthodologie --- Phosphoproteins - Metabolism - Research - Methodology --- Phosphorylation - Research - Methodology --- Protein kinases - Research - Methodology --- Phosphoprotein phosphatases - Research - Methodology
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Management --- Industrial management --- Gestion --- Gestion d'entreprise --- Research --- Recherche --- Methodology --- Methodology. --- Management - Research - Methodology
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Methodological problems have hampered researchers' efforts to understand and control AIDS since the beginning of the epidemic. This practical book addresses these problems by using actual health research case studies to develop strategies regarding design and sampling, measurement, and analysis and modeling issues. Researchers working on both biological and behavioral aspects of the disease will find this work a singularly effective tool to improve their study designs.
AIDS (Disease) --- Psychological aspects --- Research --- Methodology --- Epidemiology --- Social aspects --- Klinische psychologie --- Acquired Immunodeficiency Syndrome. --- Research Design. --- Sex Behavior. --- specifieke problemen. --- Methodology. --- methods. --- AIDS (Disease) -- Epidemiology -- Research -- Methodology. --- AIDS (Disease) -- Psychological aspects -- Research -- Methodology. --- AIDS (Disease) -- Social aspects -- Research -- Methodology. --- Medicine. --- Public health. --- Health informatics. --- Epidemiology. --- Sociology. --- Health psychology. --- Sexual behavior. --- Sexual psychology. --- Medicine & Public Health. --- Public Health. --- Health Informatics. --- Health Psychology. --- Sexual Behavior. --- Sociology, general. --- Medical records --- Psychology, clinical. --- Data processing. --- AIDS (Disease) - Psychological aspects - Research - Methodology --- AIDS (Disease) - Epidemiology - Research - Methodology --- AIDS (Disease) - Social aspects - Research - Methodology
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Research --- Technological innovations --- Engineering --- Science --- Methodology --- Case studies --- Methodology. --- Case studies. --- Research - Methodology - Case studies. --- Technological innovations - Case studies. --- Research - Methodology - Case studies --- Technological innovations - Case studies --- Engineering - Methodology --- Science - Methodology
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Marketing research --- Marketing --- Methodology --- Problems, exercises, etc --- Recherche --- Méthodologie --- Problèmes et exercices --- Methodology. --- Méthodologie --- Problèmes et exercices --- Marketing research - Methodology.
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Biological techniques --- Microscopy --- Fluorescent probes --- Molecular probes --- Fluorescence microscopy --- Luminescence --- Chemiluminescence --- Biology --- Handbooks, manuals, etc. --- Methodology --- Research --- Fluorescent probes. --- Basic Sciences. Chemistry --- Basic Sciences. Biology --- Analytical Chemistry --- Biological Techniques --- Analytical Chemistry. --- Biological Techniques. --- BIOLOGY --- FLUORESCENCE --- Monograph --- Biology. --- Fluorescence. --- Radioactivity --- Life sciences --- Biomass --- Life (Biology) --- Natural history --- Microscopy - Handbooks, manuals, etc. --- Fluorescent probes - Methodology --- Molecular probes - Methodology --- Fluorescence microscopy - Methodology --- Biology - Research - Methodology
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Molecular biology --- Membranes (Biology) --- Research --- Methodology --- Membrane Lipids --- Membrane Proteins --- Cell Membrane --- Lipids --- Proteins --- Cellular Structures --- Amino Acids, Peptides, and Proteins --- Cells --- Chemicals and Drugs --- Anatomy --- Cytology --- Biology --- Health & Biological Sciences --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Cytology. --- Membranes (Biology) - Research - Methodology
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